Accelerated Vascularization of Silk Fibroin Scaffolds Through Immobilized Basic Fibroblast Growth Factor (bFGF)

Yu Liu, Jing Qu, Mingzhong Li


Angiogenesis of an implanted tissue is one of the most important issues in skin repairment. Porous bFGF-immobilized silk fibroin (bSF) scaffolds was fabricated by freeze drying using EDC agent crosslinking bFGF and SF with an aim of controlling bFGF delivery for the revascularization improvement. The bSF scaffolds retained a porous structure with 200~300 μm diameter and 85~91% porosity. The seeded L929 cells had notably improved growth and proliferation on bSF scaffolds, suggesting bFGF sustained release from SF scaffolds and remained bioactivity. After implantation into the skin defect of sprague-dawley (SD) rat dorsum, the bSF scaffolds revealed significantly higher wound recovery rate with complete reepithelialization and regeneration of skin appendages compared with SF scaffolds. The new vessels density and collagen deposition were also higher in bSF scaffolds after 4-week treatment, which provided the therapeutic potentials of the SF scaffolds in the regeneration of wounded tissue.


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