Quantitative Analysis on Cellular Uptake of Hydroxyapatite Nanoparticles in the Primary Osteoblasts in Vitro

Guoqiang Zhou, Sudan Liu, Wenshi Xu, Wenying Wang

Abstract


With the fast development of nanotechnology, the nanomaterials have raised questions concerning its potential toxic effects on human health. In this study, the effects of hydroxyapatite nanoparticles on the primary osteoblasts were investigated. As an indicator of membrane damage, lactate dehydrogenase was quantitatively assessed. The quantitative analysis on cellular uptake of hydroxyapatite nanoparticles could be detected by flow cytometer and inductively coupled plasma mass spectrometry, respectively. The results demonstrated that hydroxyapatite nanoparticles can enter cells through cell membrane and the nanoparticles taken up in the cells followed dose and time dependent manner. The methods could be used for the initial screening of the uptake potential of nanoparticles as an index of nanotoxicity.


DOI
10.12783/dtetr/apetc2017/11121

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