A Method for SNP Genotyping Using MoS2 and a DNA Intercalating Dye

HAI-XIA SHI, ZE-BIN DENG, LI GAO

Abstract


The genotype is an association with aerobic capacity in the human sports. Therefore, a simple and rapid method for detecting SNPs was developed in this study. SG (SYBR Green I), DNA sequences, and molybdenum disulfide (MoS2) were used in this sensing system. MoS2 quenched the fluorescence from SG intercalated into DNA duplex. The SG fluorescence signals from the matched and mismatched DNA duplex were significant different after the addition of MoS2. This method can clearly distinguish SNPs in DNA sequences.

Keywords


SNPs detection, MoS2, SYBR Green I, DNA duplex.


DOI
10.12783/dtbh/icmsb2017/17965

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