Purification of Factor VIII From Cryoprecipitation Using MacroCapTM Q Column

PENG-BO HOU, LIN FU, MIN LIU, XIAO-NING JIA, QING-HUA LIU, SHOU-DONG GUO, WEN-GANG SONG

Abstract


This article reported a method to prepare high quality of factor VIII using MacroCapTM Q. Results indicated that the specific activity of the FVIII eluate was varying between 35 and 80 IU/mg of protein. This means that this procedure could provide at least 95- to 216-fold purer FVIII compared with the original cryoprecipitation with a recovery rate approximately 60.5 %. The procedure is easily applicable to large-scale production of FVIII with acceptable recovery.

Keywords


Haemophilia, Factor VIII activity, Cryoprecipitation.


DOI
10.12783/dtbh/icmsb2017/17953

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