Preparation of Factor VIII Using an Ion Exchange Medium CaptoTM MMC

PENG-BO HOU, MIN LIU, LIN FU, XIAO-NING JIA, QING-HUA LIU, SHOU-DONG GUO, WEN-GANG SONG

Abstract


This article reported a method to prepare high quality of factor VIII using CaptoTM MMC. Results indicated that the specific activity of the FVIII eluate was varying between 40 and 90 IU/mg of protein. This means that this procedure could provide at least 102-to 230-fold purer FVIII compared with the original cryoprecipitation. The recovery rate was approximately 63.5%. The procedure is easily applicable to large-scale production of FVIII with acceptable recovery.

Keywords


Haemophilia, Factor VIII activity, Cryoprecipitation.


DOI
10.12783/dtbh/icmsb2017/17952

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